Profiling Albumin Isoforms in Human Plasma

The relative composition of albumin isoforms in human plasma samples was evaluated by direct infusion of diluted plasma (500-fold dilution) using the Xevo TQ-S micro triple-quadrupole mass spectrometer coupled with the ACQUITY UPLC^® M-Class system (Waters Corporation, Milford, CT, USA), as previously described [17 (link)]. After data deconvolution with the MaxEnt1 function on the MassLynx software (Waters Corporation, Milford, CT, USA), HSA-SH, thiolated albumin (+120 ± 2 Da, Thio-HSA), and glycated albumin (Gly-HSA, +160 ± 2 Da) were detected, and their intensities were used to calculate the relative abundances as previously described [13 (link)].