Electrophoresis of 10 µg of LVI protein as previously described15 (link) and probed with primary antibody (FPR2; 1:1000 and GPR40; 1:1000) overnight at 4 °C followed by secondary antibody (Biorad). The proteins were detected using femto chemiluminescence detection system (Pierce Chemical, Rockford, IL, USA). Densitometry was performed using Image J software (NIH, USA).
Free full text: Click here