Quantitative Gene Expression Analysis

Total RNA was isolated using RNA pure Tissue & Cell Kit (#CW0560, Cwbiotech; Beijing, China), and cDNA was synthesized using ReverTra Ace qPCR RT Kit (#FSQ-101, Toyobo; Osaka, Japan), according to the manufacturer’s protocol. Amplification and detection were performed with Power SYBR Green Master Mix (#CW0659S, Cwbiotech) and Gentier 48R System (Tianlong Technology, Xi’an, China) using the listed primers (Table S2). The observed copy numbers of target genes were normalized to internal GAPDH expression and quantified by 2^−ΔΔCt method [15 (link), 16 (link)].

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Li H., Wang Y., Feng S., Chang K., Yu X., Yang F., Huang H., Wang Y., Li X, & Guan F. (2023). Reciprocal regulation of TWIST1 and OGT determines the decitabine efficacy in MDS/AML. Cell Communication and Signaling : CCS, 21, 255.

Publication 2023

RNA pure Tissue & Cell Kit ReverTra Ace qPCR RT Kit Power SYBR Green Master Mix

Cdna Cell Gapdh Primers Sybr green Tissue

Corresponding Organization : Northwest University

Other organizations : Ministry of Education of the People's Republic of China, Shanxi Medical University, Shaanxi Provincial People's Hospital

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Copy numbers of target genes

control variables

Internal GAPDH expression

controls

Positive control: None mentioned
Negative control: None mentioned

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