To quantify cytokines released in the alveolar in vitro model, ELISA assays were performed. We quantified IL8 with SimpleStep ELISA® Kit (Abcam), TGF-β (Abcam) and IL1β with human IL-1β/IL-1F2 Immunoassay (R&D Systems) was titered using a commercial enzyme-linked immunosorbent assay kit (human IL-1β/IL-1F2 Immunoassay, R&D Systems) following the manufacturer’s instructions and the results were expressed as pg ml-1. All determinations were measure in same session. For IL8 quantification in BAL of COVID-19 patients we referred to already published quantifications (10 (link)).
Pandolfi L., Bozzini S., Frangipane V., Percivalle E., De Luigi A., Violatto M.B., Lopez G., Gabanti E., Carsana L., D’Amato M., Morosini M., De Amici M., Nebuloni M., Fossali T., Colombo R., Saracino L., Codullo V., Gnecchi M., Bigini P., Baldanti F., Lilleri D, & Meloni F. (2021). Neutrophil Extracellular Traps Induce the Epithelial-Mesenchymal Transition: Implications in Post-COVID-19 Fibrosis. Frontiers in Immunology, 12, 663303.
Corresponding Organization : Istituti di Ricovero e Cura a Carattere Scientifico
Other organizations :
Mario Negri Institute for Pharmacological Research, ASST Fatebenefratelli Sacco, University of Milan, Luigi Sacco Hospital, University of Pavia
IL8 (quantified using SimpleStep ELISA® Kit (Abcam))
TGF-β (quantified using Abcam kit)
IL1β (quantified using human IL-1β/IL-1F2 Immunoassay (R&D Systems))
control variables
None explicitly mentioned
controls
Positive control: None mentioned
Negative control: None mentioned
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