Assessing MCF-7 Cell Viability with SSF2 Compounds

The viability of MCF-7 cells was assessed following treatment with SSF2-1, SSF2-2, and SSF2-3 using an Ez-CyTox assay kit (Daeil Lab Service Co., Seoul, Korea), as described in the literature [18 (link)]. Briefly, the MCF-7 cells were seeded onto 96-well plates and incubated for 24 h at 37 °C in a humidified atmosphere containing 5% CO₂. The cells were subsequently treated with SSF2-1, SSF2-2, and SSF2-3 and incubated for the indicated durations from 0 to 24 h at 37 °C in a humidified atmosphere containing 5% CO₂. For quantifying the cell viability, the cells that had been incubated for the desired durations were incubated with EZ-CyTox reagents for an additional 30 min at 37 °C in a humidified atmosphere containing 5% CO₂, and the optical density of each well was determined by measuring the absorbance at 450 nm using a microplate reader (Molecular Device, Palo Alto, CA, USA).