Immunohistochemical Analysis of Brain Markers

Paraffin-embedded brains were sliced into 5 µm sections on positively charged slides for serotonin (S-2A) receptors, ionized calcium-binding adaptor (Iba-1) and doublecortin (DCX) immunohistochemistry. The following primary antibodies; (# sc-32538, Santa Cruz Biotechnology, Dallas, TX, USA) in concentration 1:100, (# sc-32725, Santa Cruz Biotechnology, Dallas, TX, USA) in concentration 200 µg/mL and (# sc-271390, Santa Cruz Biotechnology, Dallas, TX, USA) in concentration 1:400, respectively, were used. Protocol for IHC was performed as described by Schacht and Kern [18 (link)] for S-2A receptors, Farrag et al. [19 (link)] for Iba-1 and Sirerol-Piquer et al. [20 (link)] for DCX. The quantitative analysis of immunoreactive parts percentage (IRP%) was implemented using Image J software. Seven random microscopic fields per slide were subjected to analysis after subtraction of light background.

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Mahmoud Y.K., Ali A.A., Abdelrazek H.M., Aldayel T.S., Abdel-Daim M.M, & El-Menyawy M.A. (2021). Neurotoxic Effect of Fipronil in Male Wistar Rats: Ameliorative Effect of L-Arginine and L-Carnitine. Biology, 10(7), 682.

Publication 2021

sc-32725 sc-271390

Antibodies Brains Calcium Immunohistochemistry Light Microscopic Paraffin Serotonin

Corresponding Organization : Suez Canal University

Other organizations : Princess Nourah bint Abdulrahman University

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Variable analysis

independent variables

Immunohistochemistry protocol for serotonin (S-2A) receptors, ionized calcium-binding adaptor (Iba-1) and doublecortin (DCX)

dependent variables

Quantitative analysis of immunoreactive parts percentage (IRP%)

control variables

Paraffin-embedded brains were sliced into 5 µm sections on positively charged slides
Primary antibodies used: (# sc-32538, Santa Cruz Biotechnology, Dallas, TX, USA) in concentration 1:100, (# sc-32725, Santa Cruz Biotechnology, Dallas, TX, USA) in concentration 200 µg/mL and (# sc-271390, Santa Cruz Biotechnology, Dallas, TX, USA) in concentration 1:400
Seven random microscopic fields per slide were subjected to analysis after subtraction of light background

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