Lipidomic Analysis of KRGM Gintonin

The LPA C_18:2, LPC C_18:2, and phosphatidylcholine (PC) C_16:0–18:2/PC C_18:0–18:2 contents in the KRGM gintonin were determined using liquid chromatography with tandem mass spectrometry LC-MS-MS. The standard markers for LPA, LPC, and PC were identically prepared in high-pressure liquid chromatography (HPLC)-grade methanol, and the solutions were stored at 4 °C. An Agilent series 1100 HPLC instrument (Agilent Technologies, Santa Clara, CA, USA) and an API 2000 LC-MS/MS system (Applied Biosystems, Foster City, CA, USA) were used as previously described [15 (link),19 (link)]. The resultant data were given as mean ± relative standard deviation (%) from three different samples of KRGM gintonin.

Free full text: Click here

Lee R., Kim J.H., Hwang H., Rhim H., Hwang S.H., Cho I.H., Kim D.G., Kim H.C, & Nah S.Y. (2023). Preparation of Red Ginseng Marc-Derived Gintonin and Its Application as a Skin Nutrient. Nutrients, 15(11), 2574.

Publication 2023

Agilent series 1100 HPLC instrument API 2000 LC-MS/MS system

Gintonin High pressure liquid chromatography Liquid chromatography Methanol Ms ms Phosphatidylcholine

Corresponding Organization : Konkuk University

Other organizations : University of Seoul, Korea Institute of Science and Technology, Sangji University, Kyung Hee University, Korea Brain Research Institute, Kangwon National University

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

LPA C18:2
LPC C18:2
Phosphatidylcholine (PC) C16:0–18:2
Phosphatidylcholine (PC) C18:0–18:2

control variables

Standard markers for LPA, LPC, and PC were identically prepared in high-pressure liquid chromatography (HPLC)-grade methanol
The solutions were stored at 4 °C

controls

Positive control: Standard markers for LPA, LPC, and PC were used
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!