Cell Proliferation and Drug Sensitivity Assays
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Corresponding Organization : Office of Extramural Research
Variable analysis
- Increasing concentrations of drugs
- Cellular proliferation
- Cell viability
- Equal numbers of cells plated in triplicate
- Alamar Blue proliferation assay as per the manufacturer's instructions (Invitrogen)
- 5-bromodeoxyuridine (5-BrdU) cell proliferation assay as per the manufacturer's instructions (BioVision)
- Trypan blue exclusion assay by directly counting cells plated in triplicate using the Beckman Coulter Vi-CELL (12-Sample Carousel) Cell Viability Analyzer
- Positive control: Not specified
- Negative control: Not specified
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