In Vivo Quantification of NHO Volume

NHO volumes were measured in vivo in the Inveon PET-CT multimodality system (Siemens Medical Solutions, Inc.). Mice were anesthetized with a 2% isoflurane oxygen mixture. The parameters used were as follows: 360° rotation, 180 projections, 80 kV voltage, 500 μA current, and effective pixel size 36 μm. After 3D image reconstruction, NHO volume was quantified in the Inveon Research Workplace (Siemens Medical Solutions, Inc.) as previously described.^{20 (link),42 (link)}

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Tseng H.W., Girard D., Alexander K.A., Millard S.M., Torossian F., Anginot A., Fleming W., Gueguen J., Goriot M.E., Clay D., Jose B., Nowlan B., Pettit A.R., Salga M., Genêt F., Bousse-Kerdilès M.C., Banzet S, & Lévesque J.P. (2022). Spinal cord injury reprograms muscle fibroadipogenic progenitors to form heterotopic bones within muscles. Bone Research, 10, 22.

Publication 2022

Inveon PET-CT multimodality system Inveon Research Workplace

Isoflurane Mice Multimodality Oxygen Pet ct Reconstruction

Corresponding Organization :

Other organizations : University of Queensland, Hôpital Paul-Brousse, Université Paris-Saclay, Hôpital Raymond-Poincaré

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Variable analysis

independent variables

Isoflurane concentration (2%)

dependent variables

NHO volume

control variables

Oxygen mixture
Rotation (360°)
Number of projections (180)
Voltage (80 kV)
Current (500 μA)
Effective pixel size (36 μm)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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