Genetically Modified Mouse Strains

Rela ^flox/flox, H11^LSL-Cas9, and Cd4^cre mice were obtained from The Jackson Laboratory. Relb^−/−, OX40L-Tg, and Foxp3gfp reporter mice have been previously described (Xiao et al., 2012a (link)). Some strains were crossed with Foxp3gfp mice to genetically mark Foxp3⁺ T regulatory cells for cell sorting purposes. All animals were maintained in a specific pathogen-free facility at Houston Methodist Research Institute in Houston, TX. Animal use and care were approved by the Houston Methodist Animal Care Committee in accordance with institutional animal care and use guidelines.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Xiao X., Fan Y., Li J., Zhang X., Lou X., Dou Y., Shi X., Lan P., Xiao Y., Minze L, & Li X.C. (2018). Guidance of super-enhancers in regulation of IL-9 induction and airway inflammation. The Journal of Experimental Medicine, 215(2), 559-574.

Publication 2018

H11LSL-Cas9 Cd4cre

Animal Animal care committee Mice Ox40l Regulatory t cells Rela Specific pathogen free Strains

Corresponding Organization : Cornell University

Top 5 similar protocols

Variable analysis

independent variables

Rela flox/flox, H11LSL-Cas9, and Cd4cre mice
Relb−/− mice
OX40L-Tg mice
Foxp3gfp reporter mice

dependent variables

Not explicitly mentioned

control variables

All animals were maintained in a specific pathogen-free facility at Houston Methodist Research Institute in Houston, TX
Animal use and care were approved by the Houston Methodist Animal Care Committee in accordance with institutional animal care and use guidelines

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!