Western Blot Analysis of Exosomal Markers and Apoptotic Proteins

The assays were performed as previously described [6 (link)]. The primary antibodies used were as follows: GDF-15 (Abcam, ab128958), TSG101 (Proteintech, 28283-1-AP), Atrogin1 (Abcam, ab168372), CD9 (ABclonal, A19027), CD63 (ABclonal, A5271), CD81 (ABclonal, A5270), Bax (Proteintech, 50599-2-Ig), Bcl-2 (Proteintech, A11025), MHC (DSHB, MF20), Caspase3 (ABcolonal, A0214), Cleaved caspase3 (Cell Signaling Technology, 9661), Anti-mouse (Multi Sciences, GAM0072) and anti-rabbit (Multi Sciences, GAR0072). ECL Chemiluminescent Kit (Thermo Fisher, 03781) was used to visualize the antibody-antigen interaction and chemical luminescence of membranes was detected by Amersham Imager 600 (GE).

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Zhang W., Sun W., Gu X., Miao C., Feng L., Shen Q., Liu X, & Zhang X. (2022). GDF-15 in tumor-derived exosomes promotes muscle atrophy via Bcl-2/caspase-3 pathway. Cell Death Discovery, 8, 162.

Publication 2022

GDF-15 TSG101 Atrogin1 Bax Bcl-2 Cleaved caspase3 ECL Chemiluminescent Kit Amersham Imager 600

Antibodies Antibody Antigen Assays Bcl 2 Caspase3 Gdf 15 Luminescence Membranes Mouse Rabbit Tsg101

Corresponding Organization : Shanghai University of Traditional Chinese Medicine

Other organizations : East China Normal University

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Variable analysis

independent variables

Primary antibodies used: GDF-15, TSG101, Atrogin1, CD9, CD63, CD81, Bax, Bcl-2, MHC, Caspase3, Cleaved caspase3, Anti-mouse, Anti-rabbit

dependent variables

Antibody-antigen interaction and chemical luminescence of membranes

control variables

The assays were performed as previously described [6 (link)]

controls

Positive control: Not specified
Negative control: Not specified

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