Migratory and Invasive Abilities of Treated Cells

The migratory ability of DMSO- and coriloxin-treated cells was assessed using the protocol of the wound healing assay, as described elsewhere [34 (link)]. The cells that had migrated into the zone previously empty of cells at indicated times were counted through microscopic observation. Through the transwell membrane assay, performed using a transwell membrane (pore size 8 μm; Corning Costar, Cambridge, MA, USA) coated with Matrigel (BD Biosciences, Franklin Lakes, NJ, USA) [34 (link)], the cells’ invasion ability was examined. After 18 h of incubation, cells adhering to the polycarbonate filter’s lower surface were counted through light microscopy (magnification 200×) and subsequently photographed. We conducted the experiments in triplicate.