The ZIKV-Nanoluciferase (Nanoluc) construct ( Fig. 1A ) used in these assays was described previously34 (link). For maintenance and propagation of the plasmid containing the pCCI-SP6-ZIKV-Nanoluc, the E. coli Turbo strain (New England Biolabs) was used.
Complete amplification of the viral genome was performed using a PCR reaction with Phusion High Fidelity (Thermo Fisher) enzyme and the designed primers ZIKV-Forward (5′ CG ATT AAG TTG GGT AAC GCC AGG GT 3′) and ZIKV-Reverse (5′ T AGA CCC ATG GAT TTC CCC ACA CC 3′). The PCR product containing SP6 promoter followed by complete viral cDNA was purified with the DNA clean and concentration kit (Zymo Research). In vitro transcription was performed using the RiboMAX™ Large-scale RNA Production Systems kit (Promega), as instructed by the manufacturers.
Complete amplification of the viral genome was performed using a PCR reaction with Phusion High Fidelity (Thermo Fisher) enzyme and the designed primers ZIKV-Forward (5′ CG ATT AAG TTG GGT AAC GCC AGG GT 3′) and ZIKV-Reverse (5′ T AGA CCC ATG GAT TTC CCC ACA CC 3′). The PCR product containing SP6 promoter followed by complete viral cDNA was purified with the DNA clean and concentration kit (Zymo Research). In vitro transcription was performed using the RiboMAX™ Large-scale RNA Production Systems kit (Promega), as instructed by the manufacturers.
Free full text: Click here
