Blood serum samples of 20 μL were dissolved in 200 μL 0.1 N HClO4 with 25 pmol/mL 3,4-dihydroxybenzylamine (Sigma-Aldrich, St. Louis, MO, USA), followed by centrifugation at 2000× g for 20 min. In the prepared samples, serotonin concentrations were measured using high performance liquid chromatography with electrochemical detection (HPLC-ED), as previously described [38 (link)].
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