We assessed LRP5 or LRP6 mRNA levels in the different tumor subtypes: TNBC (n=41), HER2+/ER- (n=30), luminal A (n=29) and luminal B (n=30). Total RNA was extracted from frozen tumor samples with the RNeasy Mini Kit (Qiagen, Courtaboeuf, France) and was then processed with an RNA clean-up kit (Qiagen). The quality of the RNA was checked, and samples were then hybridized with U133 Plus 2.0 Affymetrix chips and processed in accordance with the manufacturer's instructions. The data were analyzed as described elsewhere [46 (link), 47 (link)].
Maubant S., Tahtouh T., Brisson A., Maire V., Némati F., Tesson B., Ye M., Rigaill G., Noizet M., Dumont A., Gentien D., Marty-Prouvost B., de Koning L., Mahmood S.F., Decaudin D., Cruzalegui F., Tucker G.C., Roman-Roman S, & Dubois T. (2018). LRP5 regulates the expression of STK40, a new potential target in triple-negative breast cancers. Oncotarget, 9(32), 22586-22604.
Other organizations :
Inserm, Centre National de la Recherche Scientifique, Université d'Évry Val-d'Essonne, École Nationale Supérieure d’Informatique pour l’Industrie et l’Entreprise, Institut des Sciences des Plantes de Paris Saclay, Université Paris-Sud, Sorbonne Paris Cité, Université Paris Cité
Tumor subtypes: TNBC, HER2+/ER-, luminal A, and luminal B
dependent variables
LRP5 mRNA levels
LRP6 mRNA levels
control variables
Frozen tumor samples
RNA extraction using RNeasy Mini Kit (Qiagen)
RNA clean-up using Qiagen kit
RNA quality assessment
Hybridization with U133 Plus 2.0 Affymetrix chips
Data analysis as described in references [46] and [47]
controls
No positive or negative controls were explicitly mentioned in the provided information.
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to
get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
