Zebrafish samples were prepared for immunofluorescence as previously described (Fausett & Goldman, 2006 (link); R. Ramachandran et al., 2010a (link); R. Ramachandran, Reifler, A., Parent, J.M and Goldman, D., 2010 (link)). Primary antibodies used in this study: Rabbit anti-GFP, Thermo Fisher, Cat. # A6455 (1/1000); Mouse anti-mCherry, Abcam, Cat. # ab125096 (1:500); and mouse anti-glutamine synthetase (GS), Sigma-Aldrich, Cat. #MAB302 (1/500). Secondary antibodies used were: Alexa Flour 555 Donkey anti Mouse-IgG (H+L), Thermo Fisher Cat. # A31570 (1:500); Alexa flour 555 Donkey anti Rabbit IgG (H+L), Thermo Fisher, Cat # A31572 (1:500); Alexa Flour 488 donkey anti mouse Thermo Fisher Cat. # A21202 (1:500); Alexa Flour 488 goat anti rabbit Thermo Fisher Cat. # A11008 (1:500).
We used an in situ Cell Death Fluorescein Kit (Millipore Sigma, Cat. # 11684795910) to detect apoptotic cells.
We used an in situ Cell Death Fluorescein Kit (Millipore Sigma, Cat. # 11684795910) to detect apoptotic cells.
