Larvae and juvenile zebrafish were anesthetized in 0.016% w/v tricaine methane sulfonate (Tricaine, MS-222) and immobilized in 5% methyl cellulose for imaging with a M165 FC stereo microscope and LAS Software V4.21 (Leica Microsystems). Total body length measurements and eye area were quantified using Fiji/ImageJ software [62 (link)]. Retinal and photoreceptor layer thickness were measured on cryosections prepared as described below and stained with Hoechst 33342 (1:10 000, Thermo Fisher) also using Fiji/ImageJ.
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Zebrafish Morphometrics Imaging Analysis
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Corresponding Organization :
Other organizations : Rutgers, The State University of New Jersey
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Variable analysis
independent variables
- Concentration of tricaine methane sulfonate (Tricaine, MS-222) used for anesthesia
- Total body length
- Eye area
- Retinal thickness
- Photoreceptor layer thickness
- Methyl cellulose used for immobilization
- Hoechst 33342 staining for cryosections
- Positive control: Not mentioned
- Negative control: Not mentioned
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