Astroglial Glutamate Release Mechanisms

All experiments were designed equal sizes (N = 6) per groups. The microdialysis study was compared with linear mixed effects model (LME) using SPSS for Windows (ver 25, IBM, Armonk, NY). When the F‐value of drug factor was significant, the data were analyzed by Tukey's post hoc test using BellCurve for Excel (Social Survey Research Information Co., Ltd., Tokyo, Japan). To represent the statistical significance of drug factor compared with LME and Tukey's post hoc test, the data (levels of l‐glutamate and GABA) were expressed as the area under the curve (AUC_{20‐180 min}) values.
Concentration‐dependent effects of MK801 and MEM on Sxc activity of primary cultured astrocytes were analyzed by logistic regression analysis (BellCurve for Excel). The effects of MK801 (10 μmol L⁻¹) and MEM (1 μmol L⁻¹) on cysteine‐induced astroglial glutamate release were analyzed by two‐way analysis of variance followed by Tukey's post hoc tests using BellCurve for Excel.
The data and statistical analysis comply with the recommendations on experimental design and analysis in pharmacology.30 (link)

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Okada M., Fukuyama K., Kawano Y., Shiroyama T, & Ueda Y. (2019). Memantine protects thalamocortical hyper‐glutamatergic transmission induced by NMDA receptor antagonism via activation of system xc−. Pharmacology Research & Perspectives, 7(1), e00457.

Publication 2019

SPSS for Windows BellCurve for Excel

Astroglial Cysteine Drug Factor f Gaba Glutamate Microdialysis Mk801

Corresponding Organization : Mie University

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Variable analysis

independent variables

MK801

dependent variables

Sxc activity of primary cultured astrocytes
Cysteine-induced astroglial glutamate release
Levels of l-glutamate
Levels of GABA

control variables

All experiments were designed with equal sizes (N = 6) per groups

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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