Microdissected Chromosome MLI1 Library

We generated the microdissected DNA library from 15 copies of the chromosome MLI1 (DV1 line) as was described previously (Zadesenets et al. 2016 (link); Zadesenets, Schärer et al. 2017 (link)). The initial DNA amplification of the collected chromosomes was performed using a GenomePlex Single Cell Whole Genome Amplification Kit (WGA4) (Sigma-Aldrich, USA) according to the manufacturer's protocol. Preparing the MLI1 DNA library was performed using NEBNext Ultra II DNA Library Prep kit (New England Biolabs, USA) and sequencing on an Illumina MiSeq with paired-end 300 bp reads at the “Molecular and Cellular Biology” core facility of the IMCB SB RAS (Novosibirsk, Russia).