Quantitative PCR Analysis of Ghrelin Signaling

A Takara reagent kit (Takara, Tokyo, Japan) was used to extract the total RNA [17 (link)]. The RNA was reverse-transcribed into cDNA by using a PrimeScript RT reagent kit (Takara). The expression of ghrelin, GHSR1α, GHSR1β, and mTOR RNA was detected by real-time PCR using a quantitative PCR system (ABI 7300, company, city, state, U.S.A.) with SYBR Premix. After the reaction, real-time PCR amplification and thawing curves were confirmed, and the 2^−ΔΔCt values were calculated.

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Lou J., Liu L., Zhang W., Zhou Z, & Fan Y. (2019). Differential expression of ghrelin and GHSR via the mTOR pathway during the dynamic carcinogenic process involving oral, potentially malignant disorders. Bioscience Reports, 39(12), BSR20192102.

Publication 2019

Takara reagent kit PrimeScript RT reagent kit

Cdna Ghrelin Mtor Real time pcr

Corresponding Organization : Nanjing Medical University

Other organizations : Shanghai Medical College of Fudan University, Shanghai First People's Hospital, University of Michigan–Ann Arbor, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University

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Variable analysis

independent variables

Takara reagent kit (Takara, Tokyo, Japan) used to extract the total RNA
PrimeScript RT reagent kit (Takara) used to reverse-transcribe the RNA into cDNA
Quantitative PCR system (ABI 7300, company, city, state, U.S.A.) with SYBR Premix used to detect the expression of ghrelin, GHSR1α, GHSR1β, and mTOR RNA

dependent variables

Expression of ghrelin, GHSR1α, GHSR1β, and mTOR RNA

control variables

Not explicitly mentioned

controls

Not explicitly mentioned

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