Flow cytometry was performed as described (6 (link)). Cells were incubated with anti-mouse CD16/32 (Fc Block; BD Biosciences) before staining with primary antibody or isotype controls. Ten-thousand to 50,000 events per sample were acquired using an LSRII flow cytometer (BD-Biosciences) and analyzed with Flowjo flow cytometry software (Tree Star Inc.). The following antibodies were used: CD11b-Brilliant Violet 421, Tim4-PE, CD138-APC, CD138-PE F4/80- Pacific Blue, Ly6G-APC-Cy7, Ly6C-APC-Cy7, CD80-PerCp-Cy5, CD40-PerCP-Cy5, CD206- PerCP-Cy5, CCR2-FITC, CX3CR1-FITC, CD169-APC, CCR5-APC, CD36-PE, CD36-AlexaFluor 488, and IL-10R-PE (Biolegend); Marco-FITC (Biorad); Ly6C-FITC, CD86-FITC, CD11c-FITC, I-A/I-E-PE, CD93-PE (BD Bioscience); CREB-PE and Phospho-CREB-FITC (Cell Signaling); CD11b-Pacific Blue, CD45-FITC, TLR4-PE, CD45-FITC (eBioscience). Buffers used in intracellular staining were from eBioscience. For cell-sorting, PEC from untreated B6 mice were stained with anti-CD11b, Tim4, and CD138 antibodies. CD11b+Tim4+ and CD11b+CD138+ cells were gated and sorted (FACSaria cell sorter). Forty-thousand cells/subset were collected and lysed immediately for RNA extraction.