Ex Vivo Complement Activation Post-TBI
Corresponding Organization :
Other organizations : Mario Negri Institute for Pharmacological Research, Istituti di Ricovero e Cura a Carattere Scientifico, University of Cambridge, Omeros Corporation (United States)
Variable analysis
- Time after TBI or sham injury (4 days)
- LP (Lectin Pathway) activity in mouse plasma samples, measured by C3c deposition on mannan-coated ELISA plates
- EDTA-plasma concentration (2.5% final plasma concentration)
- Incubation time on mannan-coated ELISA plates (15 min)
- Barbital buffered saline (BBS) composition (4 mM barbital, 145 mM NaCl, 2 mM CaCl2, 1 mM MgCl2, pH 7.4)
- Incubation temperature (37 °C)
- Incubation time with anti-C3c antibody (1 h 30 min)
- Incubation time with alkaline-phosphatase labeled goat anti-rabbit IgG antibody (1 h 30 min)
- Substrate solution (Sigma Fast p-Nitrophenyl Phosphate tablets)
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
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