Western Blot Analysis of HDAC3
Corresponding Organization :
Other organizations : Peking University Cancer Hospital, Peking University, Second Military Medical University, Changhai Hospital, Yale University
Variable analysis
- None explicitly mentioned
- Relative photographic density of the protein of interest (HDAC3)
- Cell lysates loaded in a 10% polyacrylamide gel for electrophoresis
- Nitrocellulose membrane transference based on the standard Western blot assay protocol
- 5% non-fat milk in the PBS for blocking incubated at 37 °C for 1 h
- Anti-HDAC3 antibody from Proteintech, USA for primary antibody incubation at 4 °C overnight
- Horseradish peroxidase conjugated secondary antibody (1/1000, Cell Signaling Technology, USA) incubated at 37 °C for 1 h
- PBS washing after secondary antibody incubation
- Signal developed by ECL detection system
- Positive control: Not mentioned
- Negative control: Not mentioned
Annotations
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