Methylation analysis was performed on DNA samples from saliva (n=450) and whole blood (n=40) using the Illumina Infinium Methylation EPIC, largely as described previously [22 (link)]. Data were adjusted for known epigenetic covariates, and surrogate variable analysis was performed via the sva inference module [23 ]. Our in-house-developed tool, CandiMeth [24 (link)], will be used to streamline the methylation analysis of gene lists of interest.
English A., McDaid D., Lynch S.M., McLaughlin J., Cooper E., Wingfield B., Kelly M., Bhavsar M., McGilligan V., Irwin R.E., Bucholc M., Zhang S.D., Shukla P., Rai T.S., Bjourson A.J., Murray E., Gibson D.S, & Walsh C. (2024). Genomic, Proteomic, and Phenotypic Biomarkers of COVID-19 Severity: Protocol for a Retrospective Observational Study. JMIR Research Protocols, 13, e50733.
Methylation analysis on DNA samples from saliva (n=450) and whole blood (n=40) using the Illumina Infinium Methylation EPIC
dependent variables
Methylation levels
control variables
Known epigenetic covariates
controls
No positive or negative controls were explicitly mentioned in the input.
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to
get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
