Quantification of Anti-Qβ Antibodies

Anti-Qβ development was assessed using an enzyme-linked immunosorbent assay. 96-well plates (Corning, product 3590) were coated with vidutolimod. Serum samples and monoclonal anti-Qβ (clone Qb5) standard were incubated overnight at 4°C, and human antibodies were detected using goat anti-human Ig-HRP (SouthernBiotech, catalog no. 2010-05). Plates were developed with 3,3′,5,5′-tetramethylbenzidine (Sigma-Aldrich, catalog no. T8665), and anti-Qβ concentrations were calculated from the standard curve generated with monoclonal anti-Qβ antibody (clone Qb5l ref. 14 (link)).

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Ribas A., Medina T., Kirkwood J.M., Zakharia Y., Gonzalez R., Davar D., Chmielowski B., Campbell K.M., Bao R., Kelley H., Morris A., Mauro D., Wooldridge J.E., Luke J.J., Weiner G.J., Krieg A.M, & Milhem M.M. (2021). Overcoming PD-1 Blockade Resistance with CpG-A Toll-Like Receptor 9 Agonist Vidutolimod in Patients with Metastatic Melanoma. Cancer Discovery, 11(12), 2998-3007.

Publication 2021

goat anti-human Ig-HRP

Anti antibody Antibodies Clone Enzyme linked immunosorbent assay Goat Human Monoclonal antibody Serum Tetramethylbenzidine

Corresponding Organization :

Other organizations : University of California, Los Angeles, University of Colorado Cancer Center, University of Colorado Denver, UPMC Hillman Cancer Center, University of Iowa

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Variable analysis

independent variables

Vidutolimod

dependent variables

Anti-Qβ concentrations

control variables

96-well plates (Corning, product 3590)
Serum samples
Monoclonal anti-Qβ (clone Qb5) standard
Goat anti-human Ig-HRP (SouthernBiotech, catalog no. 2010-05)
3,3′,5,5′-tetramethylbenzidine (Sigma-Aldrich, catalog no. T8665)

positive control

monoclonal anti-Qβ antibody (clone Qb5)

negative control

Not explicitly mentioned

Annotations

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