TNBC Cell Lines and Antibody Assays

Human TNBC cells (MDA-MB-231, BT-549, MDA-MB-468, HCC1806, and HCC70) were purchased from the American Type Culture Collection (ATCC, Manassas, VA) and cultured as per ATCC guidelines. All the model cells utilized were free of mycoplasma contamination. STR DNA profiling was used to confirm cell identity. Antibodies for GAPDH, p-S6, S6, p-Akt (S473), Akt, p-mTOR (S2448), mTOR, p-STAT3 (Y705), and STAT3 were purchased from Cell Signaling Technology (Danvers, MA). LIF and LIFR (LIFRα, CD118) antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX). β-actin antibody was obtained from Sigma-Aldrich (St. Louis, MO). The Ki67 antibody was purchased from Abcam (Cambridge, MA). LIFR knockout (KO) model cells and synthesis of EC359 were done using protocol in our earlier publication^{16 (link)}. Vorinostat, panobinostat, romidepsin, and givinostat were purchased from MedChemExpress (Monmouth Junction, NJ).