Cells were transfected with Lipofectamine 3000 (Invitrogen, L3000015, Carlsbad, CA, USA). The stably transfected HeLa cells were obtained as previously described [21 (link)].
The knockdown cell lines were transiently transfected with two different siRNA, which target MAGI3 and HPV18E6, and the sequences obtained as described previously [21 (link), 24 (link)] were synthesized by Sangon Biotech (Shanghai, China) Co., Ltd. The siRNA targeting β‐catenin was purchased from Santa Cruz (sc‐29209), and the siRNA sequences obtained were as follows: β‐catenin siRNA#1: 5′‐GAUAAAGGCUACUGUUGGAUU‐3′; β‐catenin siRNA#2: 5′‐CCACUAAUGUCCAGCGUUUUU‐3′.
The knockdown cell lines were transiently transfected with two different siRNA, which target MAGI3 and HPV18E6, and the sequences obtained as described previously [21 (link), 24 (link)] were synthesized by Sangon Biotech (Shanghai, China) Co., Ltd. The siRNA targeting β‐catenin was purchased from Santa Cruz (sc‐29209), and the siRNA sequences obtained were as follows: β‐catenin siRNA#1: 5′‐GAUAAAGGCUACUGUUGGAUU‐3′; β‐catenin siRNA#2: 5′‐CCACUAAUGUCCAGCGUUUUU‐3′.
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