Film opacity [25 (link)] was determined using the following equation based on the film absorbance at 600 nm and the film thickness:
In this equation, A represents the film absorbance at 600 nm, and x represents the film thickness (mm). The film opacity was expressed as A600/mm.
For the film appearance, using a handheld digital camera [Coolpix B500, Nikon, Tokyo, Japan], a digital image of the tested edible films was taken.