Comprehensive Identification of E. coli Isolates

A total of 104 isolates of E. coli obtained from patients hospitalized at Imam Reza hospital, an 800-bed training hospital in Tabriz city (in period of June to September 2021) were studied. The clinical samples from which bacterial strains were isolated included urine, wound, blood and tracheal aspirates. Bacterial isolates were identified to species level using conventional biochemical methods including IMViC tests (indole test, methyl red test, Voges-Proskauer reaction, citrate utilization test), urease test, motility, ONPG (O-nitrophenyl-beta-D-galactopyranoside), reactions observed on Triple Sugar Iron (TSI) agar (H2S and gas production, carbohydrate utilization pattern) [32 ].

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Haeili M., Barmudeh S., Omrani M., Zeinalzadeh N., Kafil H.S., Batignani V., Ghodousi A, & Cirillo D.M. (2023). Whole-genome sequence analysis of clinically isolated carbapenem resistant Escherichia coli from Iran. BMC Microbiology, 23, 49.

Publication 2023

Agar Bacterial Blood Carbohydrate Citrate E coli Galactopyranoside Imam Indole Iron sugar Motility Onpg Patients Strains Tracheal Urease Urine Wound

Corresponding Organization : Istituti di Ricovero e Cura a Carattere Scientifico

Other organizations : Tabriz University of Medical Sciences

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Bacterial isolates from urine, wound, blood, and tracheal aspirates
Identification and characterization of E. coli isolates using biochemical tests (IMViC, urease, motility, ONPG, TSI agar)

control variables

None explicitly mentioned

controls

No positive or negative controls were mentioned in the given information.

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