Measuring Leukocyte ARSA Activity

ARSA activity was estimated as mu/mg protein in leukocytes, using 4-nitrocatechol sulfate. In brief: 0.25 mM sodium pyrophosphate was utilized to inactivate arylsulfatase B(ARSB). Then, the amount of sulfate released was measured by the absorbance of free 4-nitrocatechol at 515 nm on a spectrophotometer (Beckmann Coulter, Brea, CA, USA), which is associated with sulfatase activity [17 ].

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Ataei Z., Nouri Z., Tavakoli F., Pourreza M.R., Narrei S, & Tabatabaiefar M.A. (2023). Novel in-frame duplication variant characterization in late infantile metachromatic leukodystrophy using whole-exome sequencing and molecular dynamics simulation. PLOS ONE, 18(2), e0282304.

Publication 2023

4 nitrocatechol sulfate Arylsulfatase b Leukocytes Nitrocatechol Protein Sodium pyrophosphate Sulfatase Sulfate

Corresponding Organization : University of Isfahan

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Variable analysis

independent variables

Inactivation of arylsulfatase B (ARSB) using 0.25 mM sodium pyrophosphate

dependent variables

ARSA activity measured as μ/mg protein in leukocytes
Amount of sulfate released, measured by the absorbance of free 4-nitrocatechol at 515 nm on a spectrophotometer

control variables

Not explicitly mentioned

controls

No positive or negative controls specified

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