Sirt3 Silencing via In Vivo-jetPEI Delivery

In vivo-jetPEI™ was used for control and Sirt3 siRNA administration, according to the manufacturer’s instructions. Briefly, control or Sirt3 siRNA (50 μg) was dissolved in a 100-µL mixture of equal volumes of in vivo-jetPEI™ and 10% glucose, and then injected into the tail vein once a week for 2 weeks. The Sirt3 siRNAs were designed and synthesized by Shanghai GenePharma Co., Ltd. (Shanghai, China) and the sequences were as follows: Sirt3 siRNA sense, 5′-GUCUGAAGCAGUACAGAAAtt-3′ and antisense, 5′-UUUCUGUACUGCUUCAGACaa-3′ (Srivastava et al. 2018 (link)); control siRNA sense, 5′-UUCUCCGAACGUGUCACGUTT-3′ and antisense, 5′-ACGUGACACGUUCGGAGAATT-3′ antisense (Tang et al. 2018 (link)).