Characterizing Soil Properties and Enzymes

After the harvest of potatoes, a total of 15 bulk soil samples (three treatments × five replicates) were collected with a 2-cm-diameter auger on 14 October 2021. For the bulk soils, 10 topsoil samples (0–20 cm) were randomly collected from each replicate plot and combined into a single sample (a replicate). Each composite sample (a replicate) was divided into two parts, where the first part was stored at –80°C before DNA extraction, and the second part was air-dried at room temperature for determining soil chemical properties [pH, total nitrogen (TN), alkali hydrolyzable nitrogen (AN), organic matter (SOM), total phosphorus (TP), and available phosphorus (AP)], enzyme activities [β-1,4-N-acetyl-glucosaminidase (NAG), β-1,4-glucosidase (β-GC), and alkaline phosphatase (ALP)].

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Li Q.M., Zhang D., Zhang J.Z., Zhou Z.J., Pan Y., Yang Z.H., Zhu J.H., Liu Y.H, & Zhang L.F. (2023). Crop rotations increased soil ecosystem multifunctionality by improving keystone taxa and soil properties in potatoes. Frontiers in Microbiology, 14, 1034761.

Publication 2023

Alkali Bulk Chemical properties Enzyme activities Glucosaminidase Glucosidase Nitrogen Phosphorus Potatoes Replicate

Corresponding Organization : Hebei Agricultural University

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Variable analysis

independent variables

Treatments (3 treatments)

dependent variables

Soil chemical properties (pH, total nitrogen (TN), alkali hydrolyzable nitrogen (AN), organic matter (SOM), total phosphorus (TP), and available phosphorus (AP))
Enzyme activities (β-1,4-N-acetyl-glucosaminidase (NAG), β-1,4-glucosidase (β-GC), and alkaline phosphatase (ALP))

control variables

Replicate plots (5 replicates)
Soil sampling depth (0-20 cm)

controls

Positive control: Not specified
Negative control: Not specified

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