The Lineweaver–Burk plot was used for the kinetic assay, the concentrations of kynuramine were between 20 and 180 μM, and the different folds of the ligands were prepared based on the IC50. The concentration of the MAO-B used was 2.5 U/mL, and the reaction was monitored using a plate reader after 10 min.
The Lineaweaver–Burk plot was calculated as:
where [S] is the concentration of MAO-B, and v and Vmax represent the enzyme reaction rate and the maximum enzymatic reaction velocity, respectively.
The Lineaweaver–Burk plot was calculated as:
where [S] is the concentration of MAO-B, and v and Vmax represent the enzyme reaction rate and the maximum enzymatic reaction velocity, respectively.
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