Genetic Lineage Tracing in Mice

Foxl1-CreERT2 mice were crossed to Porcn-ex3-7Neo-flox^{16 (link)}, Rosa-membrane-targeted dimer tomato protein/membrane-targeted green fluorescent protein (mG) ^{11 (link)}, or Rosa-YFP^{21 (link)} mice to obtain Foxl1-CreERT2;PorcnΔ, Foxl1-CreERT2;Rosa-mTmG, or Foxl1-CreERT2;Rosa-YFP mice. Non-inducible Foxl1Cre ^{10 (link)} mice were also crossed to Rosa-YFP and Rosa-mTmG reporter lines (Jackson Laboratories). The animal experiments were conducted under a protocol approved by the University of Pennsylvania Institutional Animal Care and Use Committee.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Shoshkes-Carmel M., Wang Y.J., Wangensteen K.J., Tóth B., Kondo A., Massassa E.E., Itzkovitz S, & Kaestner K.H. (2018). Subepithelial telocytes are an important source of Wnts that supports intestinal crypts. Nature, 557(7704), 242-246.

Publication 2018

Rosa-YFP Rosa-mTmG reporter lines

Green fluorescent protein Institutional animal care and use committee Membrane protein Mice Rosa Tomato

Corresponding Organization :

Other organizations : University of Pennsylvania, Weizmann Institute of Science

Top 5 similar protocols

Variable analysis

independent variables

Foxl1-CreERT2 mice crossed to Porcn-ex3-7Neo-flox mice
Foxl1-CreERT2 mice crossed to Rosa-membrane-targeted dimer tomato protein/membrane-targeted green fluorescent protein (mG) mice
Foxl1-CreERT2 mice crossed to Rosa-YFP mice
Non-inducible Foxl1Cre mice crossed to Rosa-YFP reporter line
Non-inducible Foxl1Cre mice crossed to Rosa-mTmG reporter line

dependent variables

Phenotypic and/or molecular changes in mice resulting from the genetic modifications

control variables

Animal housing and care conditions were kept constant

controls

Positive control: None specified
Negative control: None specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!