Murine Bone Marrow-Derived MSC Culture

MSCs derived from the bone marrow of C57BL/6 mice were purchased from Cyagen Biosciences, Inc. (Guangzhou, China).30 (link) The MSCs were cultured in 75 cm² polystyrene tissue culture flasks (Corning, USA) at a density of 5 ×10³ cells/cm² in DMEM/F12 medium (Hyclone, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, USA). The MSCs were passaged at a ratio of 1:3 using trypsin/EDTA solution (Hyclone, USA). All cultures were incubated in a humidified incubator containing 5% CO₂ at 37 °C.

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Xu R., Bai Y., Min S., Xu X., Tang T, & Ju S. (2020). In vivo Monitoring and Assessment of Exogenous Mesenchymal Stem Cell-Derived Exosomes in Mice with Ischemic Stroke by Molecular Imaging. International Journal of Nanomedicine, 15, 9011-9023.

Publication 2020

C57BL/6 mice FBS

Bone marrow C57bl mice Edta Fetal bovine serum Polystyrene Tissue Trypsin

Corresponding Organization :

Other organizations : Zhongda Hospital Southeast University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

None explicitly mentioned

control variables

Culture conditions: 75 cm^2 polystyrene tissue culture flasks, DMEM/F12 medium supplemented with 10% fetal bovine serum, trypsin/EDTA solution for passaging, incubation in a humidified incubator with 5% CO2 at 37 °C

controls

No positive or negative controls were explicitly mentioned in the provided information.

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