Cell Culture Conditions for Cancer Research

MDA-MB-231, BT549, MDA-MB-468 breast cancer cells, 293T, 143B (human osteosarcoma cells), SKOV3 (human ovarian cancer cells), DU145 (human prostate cancer cells), and SW1990 (human pancreatic cancer cells) were purchased from ATCC (Manasseh's, VA, USA). Cells were maintained in L-15 or in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. MDA-MB-231-LUC was cultured in Minimum Essential Medium supplemented with 10% FBS and non-essential amino acids^{39 (link)}. HUVECs (ScienCell Research Laboratories, San Diego, CA, USA) were purchased from Science Research Laboratories and cultured in complete ECM (ScienCell) supplemented with 5% FBS. CHO cells were maintained in DMEM supplemented with 10% FBS, 2 mM L-glutamine, 1 mM sodium pyruvate, and 100 μM non-essential amino acids. All cells were maintained at 37 °C under a humidified 5% CO₂ incubator. All cell lines were periodically monitored for mycoplasma contamination.