Knockdown of DAB2IP in Prostate Cancer Cell Lines

DAB2IP knockdown cell lines (LAPC4-KD and PC3-KD) were constructed by using the shRNA system (pGIPZ-lentiviral-shRNAmir from Open Biosystems, Huntsville, AL) and selected under puromycin. The cell lines were kindly provided by Dr. Jer-Tsong Hsieh (Department of Urology, University of Texas Southwestern Medical Center) [26 (link)]. LAPC4-KD cells were maintained in Iscove's Modified Dulbecco's Medium (IMDM) (Gibco, Grand Island, NY) supplemented with 5% fetal bovine serum (FBS) (Hyclone, Logan, UT). PC3-KD cells were maintained in RPMI1640 supplemented with 5% FBS. The cells were cultured in a humidified atmosphere containing 5% CO₂ [27 (link)].

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Chen Y.A., Lien H.M., Kao M.C., Lo U.G., Lin L.C., Lin C.J., Chang S.J., Chen C.C., Hsieh J.T., Lin H., Tang C.H, & Lai C.H. (2017). Sensitization of Radioresistant Prostate Cancer Cells by Resveratrol Isolated from Arachis hypogaea Stems. PLoS ONE, 12(1), e0169204.

Publication 2017

pGIPZ-lentiviral-shRNAmir

Atmosphere Cell lines Cells Fetal bovine serum Pc3 cells Puromycin Shrna

Corresponding Organization : Chang Gung Memorial Hospital

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Variable analysis

independent variables

DAB2IP knockdown

dependent variables

Cell line phenotypes (not explicitly mentioned)

control variables

Culture conditions (IMDM with 5% FBS for LAPC4-KD, RPMI1640 with 5% FBS for PC3-KD, 5% CO2 humidified atmosphere)

controls

Positive control: Not mentioned
Negative control: Not mentioned

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