DAB2IP knockdown cell lines (LAPC4-KD and PC3-KD) were constructed by using the shRNA system (pGIPZ-lentiviral-shRNAmir from Open Biosystems, Huntsville, AL) and selected under puromycin. The cell lines were kindly provided by Dr. Jer-Tsong Hsieh (Department of Urology, University of Texas Southwestern Medical Center) [26 (link)]. LAPC4-KD cells were maintained in Iscove's Modified Dulbecco's Medium (IMDM) (Gibco, Grand Island, NY) supplemented with 5% fetal bovine serum (FBS) (Hyclone, Logan, UT). PC3-KD cells were maintained in RPMI1640 supplemented with 5% FBS. The cells were cultured in a humidified atmosphere containing 5% CO2 [27 (link)].
Free full text: Click here