Drosophila Larval Filet Preparation and Staining

Larval filet preparation and staining was essentially performed as described (Han et al., 2012 (link)). The following antibodies were used: rabbit anti-phospho-Ret (1:50, Cell Signaling Technology, Danvers, MA), mouse anti-GFP (1:100, Roche Diagnostics, Mannheim, Germany), mouse anti-mys (1:200, Developmental Studies Hybridoma Bank, (DSHB), Iowa City, IO), mouse anti-mew (1:50, DSHB). Secondary DyLight or Alexa conjugated donkey antibodies were from Jackson ImmunoResearch (Westgrove, PA) and were used at 1:400–1:1000.

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Soba P., Han C., Zheng Y., Perea D., Miguel-Aliaga I., Jan L.Y, & Jan Y.N. (2015). The Ret receptor regulates sensory neuron dendrite growth and integrin mediated adhesion. eLife, 4, e05491.

Publication 2015

rabbit anti-phospho-Ret mouse anti-GFP mouse anti-mys

Antibodies Diagnostics Donkey Hybridoma Larval Mouse Rabbit

Corresponding Organization : University of California, San Francisco

Other organizations : Cornell University, Imperial College London

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Variable analysis

independent variables

Antibodies used for staining:
- rabbit anti-phospho-Ret (1:50, Cell Signaling Technology, Danvers, MA)
- mouse anti-GFP (1:100, Roche Diagnostics, Mannheim, Germany)
- mouse anti-mys (1:200, Developmental Studies Hybridoma Bank, (DSHB), Iowa City, IO)
- mouse anti-mew (1:50, DSHB)

dependent variables

Larval filet preparation and staining outcomes

control variables

Not explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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