For mNPH, each anaesthetised mouse was placed into a holder constructed from a standard polypropylene 50-mL conical centrifuge tube that was positioned in the centre of the modified solenoid coil and water jacket [37 (link)]. Treatment duration was 20 min for either control (0 kA/m) or mNPH (24 kA/m peak amplitude) groups. Higher amplitude mNPH is desirable in studies with mice to simulate the effects of off-target heating resulting from eddy currents. Mice present a significantly smaller radius and volume of tissue than do humans, and thus much less eddy current heating will be generated. To compensate, higher AMF amplitudes are needed to produce off-target heating as would be encountered in a clinical setting. From the PC3 tumour-bearing mice, six were chosen for thermometry. A single AMF compatible fibre-optic temperature sensor (FISO Technologies, Quebec, Canada) was inserted into the tumour (with the tip at the approximate centre of the tumour) of each of the six randomly selected animals to measure intratumour temperatures at 1-s intervals. These animals were used only for thermometry and not for tumour growth end points. Conversely, in seven randomly selected mice bearing LAPC-4 tumours thermometry was performed on the tumour surface. These same individuals were used for tumour growth comparisons.