Western Blot Analysis of Cellular Signaling

Western blot analysis was performed as previously described.^{15 (link),16 (link)} Primary antibodies for immunoblotting are as follows: PR (sc-7208; Santa Cruz Biotechnology), phospho-STAT2 (4441; Cell Signaling), Total STAT2 (sc-1668; Santa Cruz Biotechnology), IFIT3 (sc-393512; Santa Cruz Biotechnology), IFIT1 (14769; Cell Signaling), STAT1 (9172; Cell Signaling), Ubiquitin (3933; Cell Signaling), and beta-tubulin (2128; Cell Signaling). Densitometry to quantify immunoblots was performed using ImageJ.

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Walter K.R., Balko J.M, & Hagan C.R. (2020). Progesterone receptor promotes degradation of STAT2 to inhibit the interferon response in breast cancer. Oncoimmunology, 9(1), 1758547.

Publication 2020

phospho-STAT2 IFIT3 IFIT1 STAT1 Ubiquitin beta-tubulin

Antibodies Beta tubulin Densitometry Immunoblots Stat1 Stat2 Ubiquitin Western blot analysis

Corresponding Organization : University of Kansas Medical Center

Other organizations : Vanderbilt University Medical Center

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Variable analysis

independent variables

Primary antibodies for immunoblotting

dependent variables

Protein expression levels of PR, phospho-STAT2, Total STAT2, IFIT3, IFIT1, STAT1, Ubiquitin, and beta-tubulin

control variables

Western blot analysis protocol as previously described in references 15 and 16
Densitometry to quantify immunoblots using ImageJ

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

Annotations

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