Wild type (WT), Nlrp3−/−, Casp1/11−/−, P2x7r−/−, and Asc−/−C57BL/6 mouse strains were obtained from our Isogenic Unit (Immunology Department of Institute of Biomedical Sciences of University of São Paulo, Brazil). Casp1/11−/− mice (42 (link)) were provided by Dr. Flavell, R. (Howard Hughes Medical Institute, Yale University School of Medicine), Asc−/− mice (43 (link)) were provided by Dr. Zamboni, D. S. (University of São Paulo, Ribeirão Preto, São Paulo, Brazil), Nlrp3−/− mice (44 (link)) and P2x7r−/− (45 (link)) mice were obtained from Jackson Laboratories and used at 8–11 weeks of age. Specific pathogen free mice were fed with sterilized laboratory chow and water ad libitum. Animal experiments were performed in strict accordance with the Brazilian Federal Law 11,794 establishing procedures for the scientific use of animals, and the State Law establishing the Animal Protection Code of the State of São Paulo. All efforts were made to minimize suffering, and all animal procedures were approved by the Ethics Committee on Animal Experiments of the Institute of Biomedical Sciences of University of São Paulo (Proc.76/04/CEUA).
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