Cell Culture of Equine and Mammalian Cells

(i) RK13 cells. Rabbit kidney (RK13) cells were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA) and maintained in minimal essential medium (MEM) (Thermo Fisher Scientific) with 10% fetal calf serum (FCS; Thermo Fisher Scientific) and antibiotics.
(ii) MDCK cells. Madin-Darby canine kidney (MDCK) epithelial cells (ATCC) were maintained in MEM containing 1 mg/ml lactalbumin (Sigma-Aldrich) and antibiotics.
(iii) EREC. Primary equine respiratory epithelial cells (EREC) were isolated and cultured as described previously (26 (link), 55 (link)).
(iv) Equine monocytes, equine T lymphocytes, and equine polymorphonuclear cells. Equine peripheral blood mononuclear cells (PBMC) and polymorphonuclear (PMN) cells were isolated as described previously (47 (link), 56 (link), 57 (link)).

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Van Cleemput J., Poelaert K.C., Laval K., Vanderheijden N., Dhaenens M., Daled S., Boyen F., Pasmans F, & Nauwynck H.J. (2020). An Alphaherpesvirus Exploits Antimicrobial β-Defensins To Initiate Respiratory Tract Infection. Journal of Virology, 94(8), e01676-19.

Publication 2020

MEM lactalbumin

Antibiotics Cells Epithelial cells Equine Kidney Lactalbumin Madin darby canine kidney cells Monocytes Peripheral blood mononuclear cells Polymorphonuclear cells Rabbit Respiratory T lymphocytes

Corresponding Organization :

Other organizations : Ghent University, Princeton University, Fox Chase Cancer Center

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Variable analysis

independent variables

(i) RK13 cells
(ii) MDCK cells
(iii) EREC
(iv) Equine monocytes, equine T lymphocytes, and equine polymorphonuclear cells

dependent variables

Not explicitly mentioned

control variables

Minimal essential medium (MEM)
10% fetal calf serum (FCS)
Antibiotics
1 mg/ml lactalbumin

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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