Lipid Panel Measurement Methods

We received venous blood samples from all subjects after at least 12 h of fasting. The enzymatic methods with commercially available kits, including Tcho-1, TG-LH (RANDOX Laboratories Ltd., Ardmore, Diamond Road, Crumlin Co., Antrim, UK, BT29 4QY), Cholestest N HDL, and Cholestest LDL (Daiichi Pure Chemicals Co., Ltd., Tokyo, Japan) were used for detecting levels of serum TC, TG, HDL-C, and LDL-C, respectively, in samples. However, the immunoturbidimetric immunoassay (RANDOX Laboratories Ltd.) was needed to explore serum ApoA1 and ApoB concentrations. All determinations were performed with an autoanalyzer (Type 7170A; Hitachi Ltd., Tokyo, Japan) in the Clinical Science Experiment Center of the First Affiliated Hospital, Guangxi Medical University [37 (link)–48 ].

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Miao L., Yin R.X., Huang F., Chen W.X., Cao X.L, & Wu J.Z. (2017). The effect of MVK-MMAB variants, their haplotypes and G×E interactions on serum lipid levels and the risk of coronary heart disease and ischemic stroke. Oncotarget, 8(42), 72801-72817.

Publication 2017

Tcho-1 TG-LH immunoturbidimetric immunoassay Type 7170A

Apoa1 Apob Diamond Enzymatic Immunoassay Immunoturbidimetric Randox Serum Venous blood

Corresponding Organization : Guangxi Medical University

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Variable analysis

independent variables

No independent variables explicitly mentioned.

dependent variables

Serum total cholesterol (TC) levels
Serum triglyceride (TG) levels
Serum high-density lipoprotein cholesterol (HDL-C) levels
Serum low-density lipoprotein cholesterol (LDL-C) levels
Serum apolipoprotein A1 (ApoA1) concentrations
Serum apolipoprotein B (ApoB) concentrations

control variables

Subjects fasted for at least 12 hours prior to blood sample collection

controls

No positive or negative controls specified.

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