Whole Mount Embryo Sectioning and Staining

Whole mount in situ hybridization and immunohistochemistry protocols used in this study were previously described (Phillips et al., 2001 (link)). Whole mount stained embryos were cut into 10 µm sections using a cryostat as previously described (Vemaraju et al., 2012 (link)). The following antibodies were used in this study: Anti-Islet1/2 (Developmental Studies Hybridoma Bank 39.4D5, 1:100), Anti-GFP (Invitrogen A11122, 1:250), Alexa 546 goat anti-mouse or anti-rabbit IgG (ThermoFisher Scientific A-11003/A-11010, 1:50). Promega terminal deoxynucleotidyl transferase (M1871) was used according to manufacturer’s protocol to perform the TUNEL assay.

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Kantarci H., Gou Y, & Riley B.B. (2020). The Warburg Effect and lactate signaling augment Fgf-MAPK to promote sensory-neural development in the otic vesicle. eLife, 9, e56301.

Publication 2020

Anti-Islet1/2 Anti-GFP

Anti igg Antibodies Assay Embryos Goat Hybridoma Immunohistochemistry In situ hybridization Mouse Promega Rabbit Terminal deoxynucleotidyl transferase Tunel

Corresponding Organization :

Other organizations : Texas A&M University

Top 5 similar protocols

Variable analysis

independent variables

Whole mount in situ hybridization and immunohistochemistry protocols

dependent variables

Whole mount stained embryos
10 µm sections using a cryostat

control variables

Whole mount in situ hybridization and immunohistochemistry protocols previously described (Phillips et al., 2001)
Cutting whole mount stained embryos into 10 µm sections using a cryostat as previously described (Vemaraju et al., 2012)

positive controls

Anti-Islet1/2 (Developmental Studies Hybridoma Bank 39.4D5, 1:100)
Anti-GFP (Invitrogen A11122, 1:250)

negative controls

Alexa 546 goat anti-mouse or anti-rabbit IgG (ThermoFisher Scientific A-11003/A-11010, 1:50)

other controls

Promega terminal deoxynucleotidyl transferase (M1871) used according to manufacturer's protocol to perform the TUNEL assay

Annotations

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