The flies were raised at 25°C unless otherwise mentioned.
The following flies were used in this study:
CantonS, FRT42B, FRT2A, Gal80/II, Gal80/III, FRT80, GMR-GFP, UAS-mCD8GFP, hs-FLP, ey3.5-FLP, GMR-FLP, tub-Gal80ts, elav-Gal4, sev-Gal4, LGMR-Gal4, m∂0.5-Gal4, ro-tau-LacZ, Rh6-EGFP, PanR7-Gal4 were obtained from Bloomington Drosophila Stock Center. sens-Gal4/CyO and sens-Gal4/TM6 were gift from Bassem Hassan. sequoia5 is a previously generated Sequoia loss-of-function allele (Petrovic and Hummel, 2008 (link)). UAS-Sequoia was obtained from Jay Brenman. UAS-Capricious, UAS-CapriciousID(intracellular deletion), CapriciousC18fs FRT2A and caps-LacZnls flies were kindly provided by Akinao Nose. GMR-gogo was a generous gift from Takashi Suzuki. PanR8-Gal4 was a gift from Claude Desplan. UAS-CapriciousRNAi was obtained from VDRC (VDRC Transformant ID No. 27097). PM181-Gal4 and CadN405FRT40 (Lee et al., 2001 (link)) were used in Sequoia analysis, Early Dm8 labelling OK371-VP16AD/CyO; ortC2-Gal4DBD/TM2, adult Dm8 specific OrtC1-3 LexA DBD, OrtC2B dVP16AD/CyO (Ting et al., 2014 (link)) and syn-GRASP constructs UAS-Syb::spGFP1-10 and LexAop spGFP11::CD4/TM2 (Karuppudurai et al., 2014 (link)) were used in syb-GRASP experiments.
The following flies were used in this study:
CantonS, FRT42B, FRT2A, Gal80/II, Gal80/III, FRT80, GMR-GFP, UAS-mCD8GFP, hs-FLP, ey3.5-FLP, GMR-FLP, tub-Gal80ts, elav-Gal4, sev-Gal4, LGMR-Gal4, m∂0.5-Gal4, ro-tau-LacZ, Rh6-EGFP, PanR7-Gal4 were obtained from Bloomington Drosophila Stock Center. sens-Gal4/CyO and sens-Gal4/TM6 were gift from Bassem Hassan. sequoia5 is a previously generated Sequoia loss-of-function allele (Petrovic and Hummel, 2008 (link)). UAS-Sequoia was obtained from Jay Brenman. UAS-Capricious, UAS-CapriciousID(intracellular deletion), CapriciousC18fs FRT2A and caps-LacZnls flies were kindly provided by Akinao Nose. GMR-gogo was a generous gift from Takashi Suzuki. PanR8-Gal4 was a gift from Claude Desplan. UAS-CapriciousRNAi was obtained from VDRC (VDRC Transformant ID No. 27097). PM181-Gal4 and CadN405FRT40 (Lee et al., 2001 (link)) were used in Sequoia analysis, Early Dm8 labelling OK371-VP16AD/CyO; ortC2-Gal4DBD/TM2, adult Dm8 specific OrtC1-3 LexA DBD, OrtC2B dVP16AD/CyO (Ting et al., 2014 (link)) and syn-GRASP constructs UAS-Syb::spGFP1-10 and LexAop spGFP11::CD4/TM2 (Karuppudurai et al., 2014 (link)) were used in syb-GRASP experiments.
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