qRT-PCR Analysis of Fungal Gene Expression

The cDNA was synthesized via the PrimeScript^TM RT reagent kit (TaKaRa, Dalian, China) according to instructions from the manufacturer. An RT-qPCR was carried out in a CFX96 real-time PCR system (Bio-Rad, Hercules, CA, USA) with TB Green ^® Premix Ex Taq™ (Tli RNaseH Plus) (TaKaRa, Dalian, China). The B. cinerea actin gene BcactA (Bcin16g02020) was used as internal control. The relative expression of each gene was evaluated using the ^ΔΔCT method [22 (link)]. All primers used for the RT-qPCR analyses are listed in Table S1. The RT-qPCR assay was repeated three times, each with three biological replicates.

Free full text: Click here

Tang J., Wu M., Zhang J., Li G, & Yang L. (2021). Botrytis cinerea G Protein β Subunit Bcgb1 Controls Growth, Development and Virulence by Regulating cAMP Signaling and MAPK Signaling. Journal of Fungi, 7(6), 431.

Publication 2021

PrimeScriptTM RT reagent kit CFX96 real-time PCR system TB Green ® Premix Ex Taq™ (Tli RNaseH Plus)

Actin Assay Biological Cdna Expression gene Gene Primers

Corresponding Organization : Huazhong Agricultural University

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Relative expression of each gene

control variables

The B. cinerea actin gene BcactA (Bcin16g02020) was used as internal control

controls

Positive control: None mentioned
Negative control: None mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!