Mechanical Withdrawal Threshold Assessment

Mechanical withdrawal thresholds of spinal nocifensive reflexes were measured on the left paw using Electronic von Frey Aesthesiometer (IITC Life Science, Woodland Hills, CA, United States) with a plastic tip in an exclusive testing area (catalog number 76-0488, Harvard Apparatus, Holliston, MA, United States) 1 day before-SNL and 1, 2, 3, and 4 weeks after respective treatments, as described in our previous studies (Ji et al., 2018 (link); Shen et al., 2022b (link)). The average of six measurements per subject, taken at least 30 s apart, was calculated.

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Shen C.L., Wang R., Yakhnitsa V., Santos J.M., Watson C., Kiritoshi T., Ji G., Hamood A.N, & Neugebauer V. (2022). Gingerol-Enriched Ginger Supplementation Mitigates Neuropathic Pain via Mitigating Intestinal Permeability and Neuroinflammation: Gut-Brain Connection. Frontiers in Pharmacology, 13, 912609.

Publication 2022

Electronic von Frey Aesthesiometer

Reflexes Woodland

Corresponding Organization : Lubbock Christian University

Other organizations : Texas Tech University Health Sciences Center, Institute on Aging, Texas Tech University

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Variable analysis

independent variables

Time: 1 day before-SNL and 1, 2, 3, and 4 weeks after respective treatments

dependent variables

Mechanical withdrawal thresholds of spinal nocifensive reflexes measured on the left paw

control variables

Plastic tip used for Electronic von Frey Aesthesiometer
Measurements taken in an exclusive testing area (catalog number 76-0488, Harvard Apparatus, Holliston, MA, United States)
Average of six measurements per subject, taken at least 30 s apart

controls

No positive or negative controls explicitly mentioned

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