Quantitative Real-Time PCR Analysis

Total RNA was used to extract by RNeasy plus mini kits (Tiangen). Real-time PCR was showed as previously described [18 (link)]. 36B4 was used for internal reference. The primer sequences were displayed here. RNF181: F: cac aga cga gat aag gct cga a; R: tgg cca ggt ctg tga tca at. 36B4: F: ggc gac ctg gaa gtc caa ct; R: cca tca gca cca cag cct tc. CTGF: F: ctc gcg gct tac cga ctg; R: ggc tct gct tct cta gcc tg. CYR61: F: agc agc ctg aaa aag ggc aa; R: agc ctg tag aag gga aac gc.

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Zhou R., Ding Y., Xue M., Xiong B, & Zhuang T. (2020). RNF181 modulates Hippo signaling and triple negative breast cancer progression. Cancer Cell International, 20, 291.

Publication 2020

RNeasy plus mini kits

Ctgf Primer Real time pcr

Corresponding Organization :

Other organizations : Wuhan University, Zhongnan Hospital of Wuhan University, Second Hospital of Shandong University, Xinxiang Medical University, Hubei Cancer Hospital

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Variable analysis

independent variables

Independent variables not explicitly mentioned.

dependent variables

Relative expression of RNF181
Relative expression of 36B4
Relative expression of CTGF
Relative expression of CYR61

control variables

36B4 used as internal reference

controls

No positive or negative controls specified.

Annotations

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