Genetic Regulation of Taste Perception in Mice

The subjects were adult male C57BL/6J (B6) (Charles River; n = 58) mice, T1R3 green fluorescent protein (GFP) [26 (link)] (n = 3) mice, and T1R3-KO (n = 14) [27 (link)] mice. The T1R3-GFP mice and T1R3-KO mice were originally derived from the C57BL/6J strain at Mount Sinai Medical School via homologous recombination in C57BL/6 embryonic stem cells and were maintained in this genetic background (age: 8–16 weeks; weight: 20–32 g). We used male mice to avoid the effects of sex differences because the ADM gene is reported to be directly activated by estrogen receptors in mice [28 (link)].