Automated Production of 18F-FDG and 68Ga-FAPI

At our center, we use the Explora FDG4 module with a cyclotron (CTI RDS Eclipse ST, Siemens, Knoxville, Tennessee, USA) to automatically produce ¹⁸F-FDG (61 (link)). DOTA-FAPI-04 was obtained commercially (Jiangsu Huayi Technology Co. Ltd.) and radiolabeled with ⁶⁸Ga according to the protocol published by Lindner et al. (62 (link)). DOTA-FAPI-04 and ⁶⁸Ga solution eluted from ⁶⁸Ge/⁶⁸Ga generator (IGG100, Eckert & Ziegler) were mixed with NaAc (0.5 mL). The pH was maintained at approximately 4.5, and the mixture was heated at 100°C for 10 minutes (56 (link)). The radiochemical purities of FDG and FAPI were greater than 95%.

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Li K., Liu W., Yu H., Chen J., Tang W., Wang J., Qi M., Sun Y., Xu X., Zhang J., Li X., Guo W., Li X., Song S, & Tang S. (2024). 68Ga-FAPI PET imaging monitors response to combined TGF-βR inhibition and immunotherapy in metastatic colorectal cancer. The Journal of Clinical Investigation, 134(4), e170490.

Publication 2024

CTI RDS Eclipse ST IGG100

Corresponding Organization :

Other organizations : Fudan University Shanghai Cancer Center, Fudan University, Shanghai Medical College of Fudan University, First Affiliated Hospital of Harbin Medical University, Harbin Medical University, National Institute of Environmental Health Sciences

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Variable analysis

independent variables

Use of the Explora FDG4 module with a cyclotron (CTI RDS Eclipse ST, Siemens, Knoxville, Tennessee, USA) to automatically produce 18F-FDG
Radiolabeling of DOTA-FAPI-04 with 68Ga according to the protocol published by Lindner et al.

dependent variables

Radiochemical purity of FDG
Radiochemical purity of FAPI

control variables

PH maintained at approximately 4.5 during the radiolabeling of DOTA-FAPI-04 with 68Ga
Heating the mixture of DOTA-FAPI-04 and 68Ga solution at 100°C for 10 minutes

positive controls

DOTA-FAPI-04 obtained commercially (Jiangsu Huayi Technology Co. Ltd.)
68Ga solution eluted from 68Ge/68Ga generator (IGG100, Eckert & Ziegler)

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